(a) Gene therapy (b) Recombinant gene technology (c) Polymerase chain reaction (d) All the above, Which therapy is used for modification of germ cells (spermsand eggs)? Generation of two-color transgenic zebrafish using the green and red fluorescent protein reporter genes gfp and rfp. This was treated with anti-inflammatory drugs. The term âpromoterâ as used herein refers to the DNA elements that direct and regulate transcription.
Chen et al., 1988).
fluorescent fish having one or more chromosomally integrated expression cassettes that encode a fluorescent protein. In a particular embodiment, the fish for use with the disclosed constructs and methods is the Golden zebrafish. Polly and Molly the sheep Since Dolly, other sheep have since been cloned from adult cells, as have cats, rabbits, horses, donkeys, pigs, goats and cattle. The hybrid progeny of these matings have the benefits of the transgene for fluorescence combined with the benefits derived from these other lineages. There are many ways of introducing a foreign gene into fish, including: microinjection (e.g., Zhu et al., 1985; Du et al., 1992), electroporation (Powers et al., 1992), sperm-mediated gene transfer (Khoo et al., 1992; Sin et al., 1993), gene bombardment or gene gun (Zelenin et al., 1991), liposome-mediated gene transfer (Szelei et al., 1994), and the direct injection of DNA into muscle tissue (Xu et al., 1999). Read more about Dolly and the work of the Roslin Institute here: http://www.roslin.ed.ac.uk/public-interest/dolly-the-sheep/. Change ), Chapter 3-Human Reproduction study material XII BIOLOGY, MORPHOLOGY AND ANATOMY OF COCKROACH class xi biology, Follow The Biology Blog on WordPress.com. The next year Dolly produced twin lambs Sally and Rosie, and she gave birth to triplets Lucy, Darcy and Cotton in 2000. They have also created many transgenic animals - one at a time. In addition to the stimulation of somatic growth for increasing the gross production of animal husbandry and aquaculture, transgenic technology also has many other potential applications. Polly and Molly the sheep Since Dolly, other sheep have since been cloned from adult cells, as have cats, rabbits, horses, donkeys, pigs, goats and cattle. Chourrout et al., 1986; Penman et al., 1990; Brem et al., 1988; Gross et al., 1992), enhanced growth of transgenic fish has been demonstrated in several fish species including Atlantic salmon, several species of Pacific salmons, and loach (e.g. Sperm from these fish may be used to fertilize zebrafish eggs, using methods known to those of ordinary skill in the art and methods described herein, and thereby breed transgenic zebrafish that comprise these specific transgenic integration events. A transgenic animal is one that carries a foreign gene that has been deliberately inserted into its genome. Green fluorescent protein, ZsGreen, has been used as a transformation marker in insects (Sarkar et al., 2006), knock-in mouse model for the study of KIT expressing cells (Wouters et al., 2005), and as reporters for plant transformation (Wenck et al., 2003). For example, fish that express a fluorescence transgene may be selected by exposing the fish to light of appropriate wavelength and/or by visibly inspecting the fish and observing the expression of the transgene. The technical breakthrough which produced them made Dolly the sheep possible. The development of disease resistant animals has also been tested in transgenic mice (e.g. (a) virus (b) pesticide (c) Herbicide (d) all the above, Bt stands for – (a) Biotechnology (b) Bacteria tolerant (c) Bacillus thuringiensis (d) Bollworm toxin, Where from Bt toxin gene has been obtained? The eggs from the female zebrafish are squeezed into a 35 mm plastic Petri dish.
(c) The Functional gene is incorporated into somatic cells. The availability of such fish having modified pigmentation for transgenesis with fluorescent proteins would result in better products for the ornamental fish industry due to better visualization of the various colors. In other certain aspects, the transgenic zebrafish is further defined as a transgenic Golden zebrafish. As used herein the specification, âaâ or âanâ may mean one or more. ( Log Out / 30, 2012. However, for the ornamental fish industry the dark striped pigmentation of the adult zebrafish does not aid in the efficient display of the various colors that are currently available on the market. Zoology 2.
(a) Transomics – transfection – micro infection – electro portion – retroviralvectors (b) Micro injection – transfection – electro portion – retroviral vectors – transomics (c) Transfection – micro injection – transomics – electro portion – retroviral vectors (d) None of these, one of the following is transgenic organisms (a) Holly sheep and tomato (b) Dolly sheep and subabul (c) Molly sheep and banana (d) B T cotton and tomato (Flaur saur), Transgenic plants are produced by using Ti Plasmids from the (a) Agrobacterium tumefaciens (b) E. coli (c) Bacteriophage (d) Agrobacterium varians, Vaccine is a …… (a) collection of antibiotics (b) collection of life saving drugs (c) collection of killed disease causing bacteria and virus (d) collection oflysins, The typical machine for production of bio-products through microbial is (a) sterilized glass ware (b) microprojectile (c) autoclave (d) Fermenter, The foods made from genetically modified crops required to Pass human testing because (a) they may cause allergies (b) they may alter genes (c) they may cause mutations and releasetoxins (d) all above, Tissue culture technique has been biotechnologically successful in production of – Zebrafish embryos are easily accessible and nearly transparent. More recently, Lamason et al. ROYAL BANK OF CANADA, ONTARIO, Free format text:
Photograph reproduced with kind permission of Murdo Macleod. (a) plants (b) virus (c) Bacteria (d) Fungi, In which of the following plant Bt toxin gene is expressed?
Development of expression vectors for transgenic fish.
More specifically, it will be apparent that certain agents that are both chemically and physiologically related may be substituted for the agents described herein while the same or similar results would be achieved.
Transgenic sheep produce 5 g l −1 of recombinant fibrinogen for use as a tissue sealant and 0.4 g l −1 of recombinant activated protein C, … The difference with Dolly was that it is so much more difficult to clone from an adult cell.
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